profinity exact™ Search Results


profinity exact purification resin  (Bio-Rad)
93
Bio-Rad profinity exact purification resin
( A ) SDS-PAGE analysis of overexpressed and <t>Profinity</t> <t>eXact</t> purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).
Profinity Exact Purification Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/profinity+exact%E2%84%A2/Profinity+eXact+Purification+Resin/pmc09786865-95-7-11
Average 93 stars, based on 1 article reviews
profinity exact purification resin - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
profinity exact cartridges  (Bio-Rad)
93
Bio-Rad profinity exact cartridges
( A ) SDS-PAGE analysis of overexpressed and <t>Profinity</t> <t>eXact</t> purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).
Profinity Exact Cartridges, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/profinity+exact%E2%84%A2/Bio-Scale+Mini+Profinity+eXact+Cartridges/pmc03977194-372-5-10
Average 93 stars, based on 1 article reviews
profinity exact cartridges - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
profinity exact ppal supercoiled expression vector  (Bio-Rad)
92
Bio-Rad profinity exact ppal supercoiled expression vector
( A ) SDS-PAGE analysis of overexpressed and <t>Profinity</t> <t>eXact</t> purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).
Profinity Exact Ppal Supercoiled Expression Vector, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/profinity+exact%E2%84%A2/Profinity+eXact+pPAL+Supercoiled+Expression+Vector+Kit/pmc06044570-83-7-12
Average 92 stars, based on 1 article reviews
profinity exact ppal supercoiled expression vector - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
profinity exact mini spin column  (Bio-Rad)
93
Bio-Rad profinity exact mini spin column
( A ) SDS-PAGE analysis of overexpressed and <t>Profinity</t> <t>eXact</t> purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).
Profinity Exact Mini Spin Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/profinity+exact%E2%84%A2/Profinity+eXact+Mini+Spin+Columns/pmc06044570-101-21-26
Average 93 stars, based on 1 article reviews
profinity exact mini spin column - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
bio scale mini profinity nickel cartridge  (Bio-Rad)
93
Bio-Rad bio scale mini profinity nickel cartridge
( A ) SDS-PAGE analysis of overexpressed and <t>Profinity</t> <t>eXact</t> purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).
Bio Scale Mini Profinity Nickel Cartridge, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/profinity+exact%E2%84%A2/Bio-Scale+Mini+Profinity+eXact+Cartridge/pmc06198961-58-5-10
Average 93 stars, based on 1 article reviews
bio scale mini profinity nickel cartridge - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
synthetic gene coding for n-terminal profinity exact tagged mature hlif  (GenScript corporation)
90
GenScript corporation synthetic gene coding for n-terminal profinity exact tagged mature hlif
( A ) SDS-PAGE analysis of overexpressed and <t>Profinity</t> <t>eXact</t> purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).
Synthetic Gene Coding For N Terminal Profinity Exact Tagged Mature Hlif, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/profinity+exact%E2%84%A2/synthetic+gene+coding+for+n+terminal+profinity+exact+tagged+mature+hlif/pm23628981-37-1-22
Average 90 stars, based on 1 article reviews
synthetic gene coding for n-terminal profinity exact tagged mature hlif - by Bioz Stars, 2026-09
90/100 stars
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profinity exact mini spin purification starter kit  (Bio-Rad)
N/A
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers
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profinity exact antibody reagent  (Bio-Rad)
N/A
Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins
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Image Search Results


( A ) SDS-PAGE analysis of overexpressed and Profinity eXact purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).

Journal: Microorganisms

Article Title: Molecular Cloning and Characterization of a New Family VI Esterase from an Activated Sludge Metagenome

doi: 10.3390/microorganisms10122403

Figure Lengend Snippet: ( A ) SDS-PAGE analysis of overexpressed and Profinity eXact purified Est6 in E. coli BL21 Star (DE3). Samples were run on a 4–12% NuPAGE Novex Bis-Tris mini gel. Lane M, protein molecular mass ladder (Fermentas); lane 1, total soluble cell protein; lane 2, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 3, Est6 in 50 mM Tris-HCl buffer (pH 8.0). The arrows indicate the Profinity eXact tagged Est6 and tag-free Est6, respectively. ( B ) Native PAGE analysis of purified Est6. Samples were run on a NativePAGE Novex 3–12% Bis-Tris gel. Lane M, native protein mass ladder (NativeMark unstained protein standard, Invitrogen). Lane 1, Est6 in 50 mM sodium phosphate buffer (pH 7.0); lane 2, Est6 in 50 mM Tris-HCl buffer (pH 8.0). ( C ) Zymogram of purified Est6 on an agarose gel containing 1% tributyrin. The sample order is as same as that in ( B ).

Article Snippet: The recombinant protein was purified with a Profinity eXact purification resin (Bio-Rad) in accordance with the manufacturer’s protocol.

Techniques: SDS Page, Purification, Clear Native PAGE, Agarose Gel Electrophoresis